FastQCFastQC Report
Mon 27 Feb 2023
SRR3127355.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127355.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980380
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240571.2147668629252972No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC49200.24843716862420345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78850.085.3931961
ATAGCGG3900.073.5441
AGTAGGG30350.073.257622
GAATAGG11950.073.185841
CTACGAC8800.073.17935
TAGGGTA7200.073.119954
ACGGGTA2200.070.508524
GTAGGGT7350.070.348643
ATAGGGC12350.069.6521453
TAGAGGG34000.069.1262
TAAGGGA15900.068.882543
AGGGTAA10600.067.8478245
ATAAGGG29850.067.555912
AGGGATG31050.067.215865
AATGCGG5700.066.8177951
AGTAAGG11400.066.8177951
AAGAGGG73900.066.72392
AGGGCAT15950.066.603665
AGAGGGC28200.066.508043
TGTAGGG17550.066.423982
Produced by FastQC (version 0.11.5)