FastQCFastQC Report
Mon 27 Feb 2023
SRR3127354.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127354.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1721435
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203071.179655345685431No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41680.24212357713186963No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC19720.11455558879655636No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG19670.1142651334497091No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG19550.11356804061727571No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60750.085.034371
ATAGCGG3050.078.885471
GAATAGG9200.075.89281
CTACGAC7800.075.309275
TAGGGTA6600.073.5811844
ACGGGTA2200.072.8668064
CGTAGGG5050.072.824362
ACGGGAT5000.072.609634
TAAGGGA13150.071.709673
ATAGGGC9000.071.247543
TAGGGCA7050.069.553294
TAGAGGG29500.069.5249562
TCCGCTA8500.068.822341
AGTAGGG27700.068.7662052
AGGGTAA10000.068.609765
AGGGAAT16100.068.592255
AGGGATG26850.068.2579655
AGGGCAT17350.068.2549445
ATAGGGA13900.068.179653
AGTAAGG10250.068.1184161
Produced by FastQC (version 0.11.5)