FastQCFastQC Report
Mon 27 Feb 2023
SRR3127353.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127353.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742265
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197651.1344428086427725No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40680.23348916496629388No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG20580.11812209968058819No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC19610.11255463434092976No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG19140.10985699649594062No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59300.086.351231
TAGGGTA5750.072.741194
TAGGGCA8450.070.076454
AGTAGGG27600.070.0229342
TAGAGGG29800.069.902872
ATAGGGC9750.069.891063
AGGGCAT15750.069.82225
ATAGCGG2900.069.785431
GAATAGG10550.069.147031
AGGGATG27500.068.870075
TAAGGGA13600.068.765783
TGTAGGG13200.068.040422
AGTAAGG9800.067.7153851
CGAAGGG16600.067.701362
ACGGGAT5150.067.5278244
GAGGGAT20400.067.498744
AGGGAAT17150.067.136735
CTACGAC7350.066.497335
AATAGGG26450.066.489562
GTAGGGT6650.066.430033
Produced by FastQC (version 0.11.5)