FastQCFastQC Report
Mon 27 Feb 2023
SRR3127352.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127352.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570641
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199181.2681446619564878No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22700.14452697974903242No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG16440.10467064084026839TruSeq Adapter, Index 22 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG16380.10428863120216522TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC16220.10326993883389012No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58700.084.3464661
CGTAGGG6000.081.474252
GTACGGG3150.076.1023252
TAGGGCA8450.073.983224
ATAGGGA17150.072.630673
AGGGCAT15200.071.1250465
GAATAGG11000.070.529031
ATAACGG1400.070.529021
AGTAGGG26600.069.799732
AATGCGG3100.069.7706451
CTACGAC4000.069.331465
ATAGGGC10600.069.1762543
TATAGGG13900.068.646682
AGGGATG23700.068.622425
TAGAGGG29850.067.7114942
AATAGGG25850.066.915332
CGAAGGG16900.066.7517852
TACGGGG7850.066.464793
ATGAGGG26000.066.16772
TAGGGTA5350.065.893994
Produced by FastQC (version 0.11.5)