FastQCFastQC Report
Mon 27 Feb 2023
SRR3127351.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127351.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1384321
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165221.1935093088958415No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG22090.1595728158425683TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC20960.15140996921956684No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC19780.14288593469289276No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG19640.1418746085626094TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT48200.084.424961
CGTAGGG5100.078.5310062
ATAACGG1350.076.841531
ATAGGGC8200.074.7002263
ACGGGTA1350.073.295614
GTACGGG2650.072.900482
AATGCGG3450.072.437571
TAGGGCA6550.071.217434
GAGGGAT15750.071.201454
AGGGATG20200.071.1894765
TAGGGTA5300.071.122424
TACGCGG800.070.729131
AGGGCAT13050.070.581575
AGTAGGG23750.070.2315142
TGTAGGG9950.070.085972
GAATAGG8700.068.29021
AGTAAGG9700.068.055521
AAGAGGG48800.067.5881652
ATGAGGG21900.067.3430252
ATAGGGA13750.067.165433
Produced by FastQC (version 0.11.5)