FastQCFastQC Report
Mon 27 Feb 2023
SRR3127349.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127349.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3718296
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT446641.2011953862737126No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45590.12260992669760556No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT153400.084.542551
CGTAGGG11100.073.686282
GAATAGG25100.069.8781741
AGGGATG57300.068.992485
ACGGGTA4550.068.185644
ATAGGGA35800.067.621473
TAGAGGG69200.067.521282
AGTAGGG65150.067.100982
ATAGAGG26450.066.3116151
ATAGCGG10750.065.61291
GTACGGG6850.065.1918642
TAAGGGA36100.064.976113
AGAGGGC57350.064.506233
AAGAGGG144400.064.4878162
TATAGGG34900.064.112382
AGGGAAT37250.063.979685
GTAGGGA28350.063.6705443
TAGGGAT24400.063.5747264
AATAGGG59400.063.5461852
AGGGCAT36950.063.48145
Produced by FastQC (version 0.11.5)