FastQCFastQC Report
Mon 27 Feb 2023
SRR3127348.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127348.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3210823
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT374941.1677379911630135No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38270.11919062495814936No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT125550.085.599631
GAATAGG21400.072.057341
AGGGATG47400.071.878665
CGTAGGG8200.070.1228562
TAGGGAT21300.069.701894
ATAGCGG8000.069.5562741
TAAGGGA28750.069.5090943
TAGGGCA15900.069.3637164
ATAGGGA30450.069.343273
AGTAGGG63300.069.245712
TAGAGGG57250.068.001452
GGTAAGG21450.067.7123261
AGTAAGG18200.067.366851
ATAGGGC19500.067.192973
TAGGGTA10050.067.0630954
CGAAGGG33600.065.9283452
ATAGAGG23150.065.184331
GTAGGGT14250.065.1575853
TATAGGG28950.065.12162
GTACGGG6950.065.102852
Produced by FastQC (version 0.11.5)