FastQCFastQC Report
Mon 27 Feb 2023
SRR3127347.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127347.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3262738
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359361.1014062422419453No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39270.12035903587722949No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT117250.085.42491
AGTAGGG58500.070.343632
GAATAGG19950.070.3070761
ATAGCGG8400.070.041661
TAGGGCA15950.069.831914
AGGGATG51000.069.020475
TAGAGGG59400.067.4567952
CGTAGGG8850.066.957592
AAGGGTA21550.066.951054
ATAGGGA27550.066.869913
AGAGGGC51350.066.628073
AGTAAGG19050.066.216391
ATAGAGG22050.065.959231
TAGGGTA9700.065.892114
GTAGGGA24000.065.5993963
TAGACGG4600.065.48591
AGGGACT41700.065.254255
ATAGGGC17850.065.0316853
ACTAGGG16300.064.918352
CGAAGGG34900.064.817492
Produced by FastQC (version 0.11.5)