FastQCFastQC Report
Mon 27 Feb 2023
SRR3127346.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127346.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2673736
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296961.1106556518669009No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29870.11171633998270585No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92250.084.711121
ATAGCGG4750.081.17011
GTACGGG6100.077.831182
AGGGATG46650.073.558575
AGTAAGG20600.072.126631
TAGGGCA15500.071.571874
CGAAGGG24900.070.982382
TAGAGGG46800.070.912192
ATAGGGC17550.070.443473
AGTAGGG51550.070.396442
AGGGCAT26700.069.718175
CGTAGGG8950.069.328682
AGAGGGC40550.069.090363
TATAGGG22500.068.7346042
GTAGGGA21100.068.3943
ATAGGGA27850.068.189613
ACGGGTA2900.068.0790254
TAGCGGG15200.068.036382
GAATAGG19950.067.877431
AAGAGGG105600.067.082832
Produced by FastQC (version 0.11.5)