FastQCFastQC Report
Mon 27 Feb 2023
SRR3127345.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127345.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316590
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248161.0712296953712137No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26190.11305410107097069No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG23460.10126953841637924Illumina PCR Primer Index 7 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72650.085.480381
ATAGCGG4800.081.5367051
ATAGGGC17100.076.345543
GTACGGG4750.074.416232
AGGGATG40450.074.005335
AGTAGGG41000.073.339322
ATAGGGA23800.072.675693
ACGGGTA3000.072.2664264
TAGGGTA8550.071.660084
AGGGTAC13250.071.643645
TAGGGCA14750.071.574124
AGTAAGG18100.070.339851
CGTAGGG9050.069.784062
AGATAGG15200.069.4897461
GTAGGGA17200.069.325353
TAGAGGG41050.069.231572
TAAGGGA19000.069.207093
TAGCGGG14200.068.372092
TAGGGAT17500.068.136924
CGAAGGG19800.067.6010062
Produced by FastQC (version 0.11.5)