FastQCFastQC Report
Mon 27 Feb 2023
SRR3127344.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127344.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366727
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240881.017776870758647No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26520.11205348145350098No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG24650.1041522744279336Illumina PCR Primer Index 7 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69950.084.9718861
GTACGGG5850.075.566362
ATAGCGG4850.073.744811
TAGGGCA13650.072.303394
CGTAGGG8850.072.269022
AGGGATG37150.071.729245
AGGGTAC10600.071.382545
AGTAGGG44550.071.254552
AGAGGGC34900.070.293813
TAGCGGG12950.069.361762
TAGGGAT15200.068.9498444
ATAGGGC15750.068.929223
AGGGCAT22950.068.806365
ACGGGAT8200.068.776394
TAGAGGG42200.068.647512
GTAGGGT11350.068.321923
GGTAAGG14100.067.754251
GTAGGGA17900.067.738983
ATAGGGA23050.067.6922763
AGTAAGG17200.067.581541
Produced by FastQC (version 0.11.5)