FastQCFastQC Report
Mon 27 Feb 2023
SRR3127342.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127342.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725423
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172581.0002184971453376No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC56140.3253694891049905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60850.084.088561
AGGGATG47100.080.418585
GGATGGC40350.075.819297
GGTAAGG10200.075.362491
CGGGATG9800.075.2862855
TAAGGGA17700.074.286243
CGTAGGG5050.073.724722
AGGGAAT21850.073.555815
AAGGGAT45000.072.2626654
TAGGGCA10250.072.1860354
TAGGGTA6800.071.3847964
GTAGGGT7350.071.172643
GAGGGAT32150.071.094864
TAGCGGG10800.070.6917342
GCTACGA10850.070.3659744
AGGGCAT18050.070.0353555
CGAGGGA13200.069.9776843
AGTACGG2700.069.86571
TAGGGAT20200.069.525214
AGTAGGG31600.069.498632
Produced by FastQC (version 0.11.5)