FastQCFastQC Report
Mon 27 Feb 2023
SRR3127341.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127341.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755000
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171050.9746438746438747No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC55010.31344729344729344No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC17870.10182336182336182No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG17600.10028490028490028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57800.084.128711
AGGGATG47600.080.56325
CGTAGGG5550.077.964152
ATAACGG850.077.5319751
GGATGGC39600.076.782567
TAGCGGG9800.075.828262
TAGGGAT18300.072.932444
GTAGGGC9750.072.30033
CGGGATG10800.072.233375
GGTAAGG12250.071.858351
GAGGGAT34300.071.383384
AGTAGGG30250.071.365352
TAGACGG1650.071.322711
ATGCGGG9800.071.029012
AGGGCAT17400.070.7628865
TAAGGGA17900.070.361533
AAGGGAT45250.070.207194
TAGGGCA11750.069.992854
ATGGCAC39800.069.9024059
GGGATGG49850.069.3850866
Produced by FastQC (version 0.11.5)