FastQCFastQC Report
Mon 27 Feb 2023
SRR3127340.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127340.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926764
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246851.281163650556062No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21630.11226076468109224No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21270.11039234696101857No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75250.085.490961
CGTAGGG4450.079.22282
TAGGGCA9650.074.52694
TAAGGGA16800.072.466863
GACCGAT1300.072.31628
AGGGCAT17750.071.766185
ACGGGTA2850.070.920624
AGGGATG26550.070.4640355
ATAACGG2150.069.99261
AGTAGGG29550.068.559682
GTAGGGT7750.068.5370943
ATAAGGG29850.068.185582
ATAGGGC10350.068.1239553
AGTACGG2350.068.0380251
GAGGGAT23500.068.0079964
TAGAGGG37050.067.8757252
AAGGGCA30050.067.7317664
GAATAGG9500.067.321831
TAGACGG3850.067.18041
AAGAGGG69800.067.073792
Produced by FastQC (version 0.11.5)