FastQCFastQC Report
Mon 27 Feb 2023
SRR3127339.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127339.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1674630
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204751.2226581394098994No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG22640.13519404286319964No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC21280.1270728459420887No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG19310.11530905334312654No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18320.10939729970202373No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC18170.10850157945337179No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62300.086.569821
ACGGGTA1950.079.806884
CGTAGGG5000.076.396942
TAGACGG2850.074.503381
TAGGGCA9900.074.310544
ATAGGGC10000.073.567433
GTACGGG3950.072.827222
GAATAGG7150.072.593041
GTAGGGA11600.071.9575353
AGTACGG2800.070.778211
AGGGCAT14700.070.328865
TATCGGG1150.069.712732
TAGAGGG28300.069.1548542
CGGGTAT1500.068.922275
GAGGGAT20850.068.7588354
TAGCGGG8300.068.74932
AGGGATG21950.068.7224665
TAGGGTC3950.068.051674
AGTAGGG27550.067.785162
AAGGGCA23950.067.144424
Produced by FastQC (version 0.11.5)