FastQCFastQC Report
Mon 27 Feb 2023
SRR3127338.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127338.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1721722
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198871.1550645226116645No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG25110.1458423601487348No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC22720.13196090890399262No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTG20770.12063503864154608No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19180.11140009827370506No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC19140.11116777272986No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61250.084.359081
TAGGGCA10550.077.0657354
ATAGGGC10550.075.729333
ATAGGGA13050.072.025643
AGGGCAT17050.071.9422155
CGAAGGG15550.070.775212
TAGAGGG32250.070.438992
ATAGAGG12650.070.308351
ACGGGTA2150.069.948624
GTAGGGA11850.069.8010563
AGTAGGG29950.068.938752
AAGGGCA27150.068.720824
GAATAGG8300.068.602971
AGGGATG19850.068.186695
ATAAGGG22050.068.0421142
TAAGGGA13150.067.904023
GAGGGAT18750.067.675294
AGGGCAA18100.067.509125
AGAGGGC23800.067.3356553
GACCGAT700.067.138198
Produced by FastQC (version 0.11.5)