FastQCFastQC Report
Mon 27 Feb 2023
SRR3127337.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127337.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2759755
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292441.0596592813492502No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28670.1038860333616571No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99500.084.123711
TAGGGCA18450.074.644184
AGGGATG45250.073.958175
CGTAGGG8400.072.742592
TAGCGGG15300.070.043552
GAATAGG18000.070.0138241
TAGAGGG53050.069.7290952
ATAGCGG7450.069.4317251
ATAGGGC15800.069.016933
AGTAGGG48200.068.554042
GTAGGGA24650.068.4545443
ACGGGTA3400.067.739484
ATAGAGG19200.067.107471
AGGGCAT26650.067.0210345
TAAGGGA25700.066.572213
AAGGGTA20450.066.4246444
GGTAAGG15650.066.1043241
TAGGGAT20100.065.9443744
AGGGAAT31000.065.652455
AGGGTAC11750.065.604065
Produced by FastQC (version 0.11.5)