FastQCFastQC Report
Mon 27 Feb 2023
SRR3127334.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127334.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2658791
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314001.1809879001395747No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43070.1619909199331576No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30310.11399918233512901No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99250.084.1026841
GAATAGG17900.071.196441
TAGGGTA9700.070.748774
ATAGGGA27250.070.722093
GTACGGG7250.069.3718952
AGTAGGG42200.068.612942
ATAAGGG39950.068.5945362
TAGAGGG50000.068.250272
AGGGATG39850.068.17695
TAGGGCA15700.067.362884
TAAGGGA26100.067.354843
ATAGGGC16000.067.274933
GGTAAGG16250.066.8498841
GGATAGG16550.066.490551
ATAGAGG18700.066.390251
TATAGGG24100.066.11812
ATGAGGG48250.066.049592
AGATAGG15900.065.955311
CGTAGGG5500.065.8060462
GTAGGGA19500.065.5653
Produced by FastQC (version 0.11.5)