FastQCFastQC Report
Mon 27 Feb 2023
SRR3127333.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127333.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2289650
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267881.1699604743083003No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36880.16107265302557158No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24950.10896861965802634No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80850.084.782521
ATAGGGC13500.075.448443
TAGGGCA12400.072.634344
TAGGGCG3100.069.972344
GAATAGG14750.069.724461
CGTAGGG5550.068.821212
GGTAAGG11050.068.309071
GTAGGGA15400.067.977083
ATAAGGG32850.067.897862
AGGGATG33800.067.847275
ATAGGGA21200.067.841323
AATAGGG36350.067.71682
TAAGGGA20200.067.6981663
AGTAGGG36550.067.604282
TAGGGTA7900.067.449954
TATAGGG18100.066.955282
AGACGGG14650.066.628952
TAGAGGG37850.066.528182
ATAGAGG16950.066.519481
TAAACGG3000.066.046331
Produced by FastQC (version 0.11.5)