FastQCFastQC Report
Mon 27 Feb 2023
SRR3127332.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127332.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2356147
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259801.1026476701156591No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36950.1568238314502448No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26050.11056186222676259No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85700.084.341441
ATAGGGC15000.073.94163
TAGGGCG3750.072.688354
ATAGGGA22150.070.866473
GTACGGG6050.070.729892
AGTAGGG36800.070.152232
TAGGGCA14150.070.084
TAGACGG3100.069.8275451
AGGGATG33300.069.718945
GAATAGG15750.069.61551
GGTAAGG13300.068.640541
TAAGGGA20250.067.071943
TATAGGG20550.066.588332
AGAGGGC36050.066.486423
AGGGAAT22150.065.986445
CGTAGGG6800.065.694912
AATAGGG38000.065.461972
AAGAGGG88400.065.375732
ATAGAGG15500.065.273571
ATAAGGG34400.065.20442
Produced by FastQC (version 0.11.5)