FastQCFastQC Report
Mon 27 Feb 2023
SRR3127331.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127331.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2701911
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278511.0307889490068325No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92050.084.54561
CGTAGGG10250.076.1258542
TAGAGGG55150.070.316372
GGTAAGG18600.070.029921
TAGCGGG17550.069.90552
AGGGATG45550.069.7599645
TAGGGCA12850.069.136384
AGGGTAC12000.068.157865
AAGGGTA19700.067.764174
GAGGGAT39950.067.53724
AGGGCAT24800.066.906915
ATAGGGA25950.066.840083
TAAGGGA22000.066.0212553
AAGAGGG100400.065.498592
AGGGACT34450.065.220885
AGTAAGG15950.065.1551061
ATAAGGG38850.065.093742
AGAGGGC40650.064.870953
AGTAGGG45950.064.856232
AGAGGGA69450.064.772053
Produced by FastQC (version 0.11.5)