FastQCFastQC Report
Mon 27 Feb 2023
SRR3127330.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127330.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2337190
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233150.997565452530603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77050.085.358061
CGTAGGG8300.078.969242
GTAGGGT8400.072.415533
GGTAAGG14750.071.96891
ATAGGGA21050.071.459483
TAGGGTA7050.070.898664
AGGGATG37200.070.741985
TAGGGCA9800.070.7314454
AGTACGG2500.069.825831
AGGGTAC9100.069.714725
TAGAGGG44900.069.208642
ATGCGGG10750.068.86722
AATGCGG4900.068.3623051
TAGCGGG16400.067.8561862
AGTAGGG38050.067.788172
AGATAGG12950.067.3994451
AGGGACT31700.066.5609055
GATAGGG32700.066.477462
AAGGGTA14650.066.3056954
GTACGGG5650.065.9325562
Produced by FastQC (version 0.11.5)