FastQCFastQC Report
Mon 27 Feb 2023
SRR3127329.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127329.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2379484
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226680.9526435143081441No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79000.085.443041
CGTAGGG7300.074.0876542
AGGGATG36600.072.805215
ATAGGGA21250.072.097333
AGTACGG2200.070.60671
TAGACGG2600.070.606691
TAGAGGG46550.069.4077452
CGAAGGG23400.068.132562
TAGGGTA8000.066.969234
GGTAAGG14350.066.916451
ATGCGGG10150.066.721742
TAGGGCA9800.066.657534
GTAGGGT9400.066.494283
TAGGGAT18100.066.469414
TAGCGGG14250.066.006392
AAGAGGG81750.065.870142
AGTAAGG14000.065.5633541
ATAGAGG16900.065.453931
GATAGGG32900.065.040882
AGGGTAC10000.064.8544165
Produced by FastQC (version 0.11.5)