FastQCFastQC Report
Mon 27 Feb 2023
SRR3127328.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127328.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3116996
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348451.1179032632701484No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT119450.084.833141
TAGGGCA20550.074.795094
ATAGGGC19500.071.832143
TAGAGGG59500.071.572852
ATAGGGA30550.070.621773
ATAGCGG7350.070.3736041
AGTAGGG50250.070.342682
CGTAGGG9500.070.258992
CGAAGGG34550.070.200272
AGGGATG50550.069.832235
AGATAGG17950.068.372341
GATAGGG49150.066.274572
TGAAGGG58550.066.231442
GAATAGG19000.066.078791
GTACGGG6900.066.078442
AGGGTAT19700.065.137875
AAGAGGG118650.064.890822
ATAGAGG26000.064.746171
AAGGGTA22800.064.7339254
TAGCGGG19950.064.557212
Produced by FastQC (version 0.11.5)