FastQCFastQC Report
Mon 27 Feb 2023
SRR3127327.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127327.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2655529
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290311.0932285055068123No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94150.085.486221
ATAGCGG6200.074.571321
ATAGGGC15750.073.642653
TAGGGCA16300.071.736284
GAATAGG14350.071.670761
CGAAGGG30450.071.381962
AGTACGG3250.071.129561
AGTAGGG40200.070.020162
AGGGATG43700.069.037785
TAGAGGG47450.068.960132
CGTAGGG5800.068.2851262
GTACGGG5900.067.926892
GGTAAGG16000.067.5231861
TAGCGGG15750.067.356082
ATAGGGA27350.067.060553
GATAGGG37450.066.474782
TAAGGGA23150.066.192253
AATAGGG42250.066.064762
AGAGGGC40400.065.938933
GAGGGAT39450.065.734074
Produced by FastQC (version 0.11.5)