FastQCFastQC Report
Mon 27 Feb 2023
SRR3127326.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127326.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737205
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283151.0344493744531373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT91900.084.6114041
ATAGCGG7200.077.1407551
CGAAGGG28550.071.985392
AGTAAGG16950.070.533971
ATAGGGC15550.070.117283
AGGGATG44550.070.046725
TAGGGCA14150.069.08354
ACGGGAT8100.069.0445564
AGTAGGG42550.068.747972
ATAGGGA24900.068.702023
TAGAGGG48650.068.054822
AGATAGG14500.067.8441851
GTAGGGA21100.067.488173
GAGGGAT38850.067.259134
GTAGGGT10850.067.138163
GAATAGG16350.067.0768361
CGTAGGG8300.066.861012
TAAGGGA25950.066.4654853
TGAAGGG52500.066.109842
GATAGGG39850.065.8529052
Produced by FastQC (version 0.11.5)