FastQCFastQC Report
Mon 27 Feb 2023
SRR3127325.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127325.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1828748
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208331.139194684013325No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC18700.10225575092905091No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71400.085.015081
CGTAGGG5700.075.870692
TAGACGG2900.072.95961
AGGGATG39700.071.516695
TAGGGCA10350.071.305064
CGAAGGG18700.071.138692
ATAGGGA21150.070.0101853
GAATAGG12500.069.587251
TAGAGGG35800.068.803312
TATAGGG18250.068.7716452
AAGGGAT39850.067.236884
AGTAGGG30450.067.152622
GAGGGAT33400.067.132524
AGGGCAT19750.066.64265
GTAGGGA17050.066.443683
AGATAGG11900.066.378931
ACGGGTA2550.066.3625954
TAGGGAT18000.066.331874
GGTAAGG11800.066.144551
ATAGGGC10900.065.982073
Produced by FastQC (version 0.11.5)