FastQCFastQC Report
Mon 27 Feb 2023
SRR3127324.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127324.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558256
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177381.138323869762093No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT56250.087.3291551
AGGGATG34150.074.310355
TAGGGCA7750.072.955164
CGTAGGG4400.072.816992
ATAGGGA16250.071.037753
AAGGGAT35150.070.64184
TAGACGG2950.070.3144761
GAGGGAT26300.070.227444
TGTAGGG13500.070.1517942
CGAAGGG15900.069.934492
GAATAGG11250.069.5616151
AGTAGGG27950.069.115992
ATGCGGG8050.069.0657352
GTAGGGT4650.067.888833
TAGGGCG1950.067.6554
GTAGGGA15550.067.569543
ATAGCGG4400.067.499721
GGATGGC23150.067.395887
ATAGGGC9250.067.237053
TAGAGGG29950.067.017662
Produced by FastQC (version 0.11.5)