FastQCFastQC Report
Mon 27 Feb 2023
SRR3127323.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127323.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578898
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170231.0781570437102335No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC16360.1036165730781849No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60900.085.399591
CGTAGGG3650.081.178642
ATAGGGA16050.073.790963
GAATAGG10650.073.361681
AGGGATG30900.073.006325
ATAGGGC8000.072.259143
TAGGGAT13450.071.981784
AGTAGGG27550.069.651852
TAGCGGG8800.069.47922
GAGGGAT27350.069.422744
ATAAGGG20550.068.888832
AGGGATC16050.068.2273565
TAGAGGG30200.067.5891342
GTAGGGA14400.067.559363
AGACGGG11100.067.370262
AAGGGAT33350.067.361184
ATGCGGG8600.066.719922
AGGGATT21650.065.992325
AGAGGGC25300.065.945563
GTAGGGT5150.065.705683
Produced by FastQC (version 0.11.5)