FastQCFastQC Report
Mon 27 Feb 2023
SRR3127321.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127321.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2617063
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT395071.509593005594439No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT129600.087.597941
AGTAGGG48750.070.9772952
GTAGGGT9300.070.4579243
TAGGGCA15850.069.001214
ATAGGGC16200.068.965413
AGTAAGG17750.068.821511
ATAGCGG7200.068.1276861
TAGAGGG48250.068.097862
AGGGATG36350.067.4840555
TAGGGTA8950.067.41944
TAAGGGA20850.066.924283
GATAGGG37300.066.477542
AGATAGG16700.066.370361
ATAAGGG38050.066.2822342
AGAGGGC39900.066.044543
AGGGCAT22950.065.933825
AAGAGGG98750.065.44832
TAGCGGG16100.065.2945562
GAATAGG15700.065.1902851
ATAGGGA21500.065.120243
Produced by FastQC (version 0.11.5)