FastQCFastQC Report
Mon 27 Feb 2023
SRR3127320.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127320.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2686599
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381681.4206809427086067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT122200.087.48921
ATAGAGG17750.072.140491
TAGGGCA15700.071.242894
ATAGGGC16200.070.784643
AGTAGGG49400.070.264062
TAGAGGG48650.070.090482
TAGGGTA8250.070.067314
TAAGGGA22700.069.3558653
AGATAGG16400.068.893131
ATAAGGG38100.068.265932
AGTAAGG17650.067.748161
ACGGGTA2500.067.674764
AGAGGGC42600.067.295263
GAATAGG17700.067.024841
GTAGGGT10750.066.887853
CGTAGGG6050.066.8572
TAGCGGG13800.066.8006742
AGGGATG39550.066.781175
GATAGGG40200.066.220772
AAGAGGG102650.066.208342
Produced by FastQC (version 0.11.5)