FastQCFastQC Report
Mon 27 Feb 2023
SRR3127319.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127319.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589975
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299931.1580420660431086No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC70080.2705817623722237No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30010.11586984430351645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93450.085.8953551
CGTAGGG6600.084.038332
ACGGGTA3100.075.81374
TAGGGCA16250.075.78574
AGGGATG48100.075.050835
ATAGGGC16700.070.928933
GAGGGAT38750.070.23384
ATAGGGA27250.070.032383
GGATGGC45050.069.176427
AAGAGGG96000.069.08682
TAGAGGG48600.068.959262
CTACGAC13250.068.821675
AGAGGGA64000.067.789283
AGAGGGC37000.067.7118763
AGTAGGG43700.067.548792
TCCGCTA13750.067.0299451
GTAGGGT10150.066.686173
TAAGGGA25950.066.6576163
GAAGGGC34000.066.635773
AGGGCAT25900.066.423335
Produced by FastQC (version 0.11.5)