FastQCFastQC Report
Mon 27 Feb 2023
SRR3127318.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127318.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231071
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244761.0970515953996982No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC60710.27211146574896095No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG27850.12482794137882658No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27660.12397633244302848No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC25090.11245720104828577No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG24120.1081095133234218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75350.086.286731
CGTAGGG6600.078.5463942
TAGGGCA12300.074.714874
AGGGATG44150.074.402265
CTACGAC12150.073.101325
ACGGGTA2450.073.096244
ATAGCGG5250.072.7425841
GAGGGAT31500.072.41234
CGAAGGG22800.071.931962
GGTAAGG13050.071.896141
ATAGGGA23100.071.20183
GGATGGC36800.069.596657
TAGAGGG39100.069.305652
AGAGGGC30200.068.819133
AGTAAGG13450.068.706341
AGGGAAT24750.068.544355
AGAGGGA55350.068.201263
AAGGGCA35700.067.853534
GAATAGG12800.067.775211
TCCGCTA13100.067.6627351
Produced by FastQC (version 0.11.5)