FastQCFastQC Report
Mon 27 Feb 2023
SRR3127317.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127317.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280620
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237801.042698915207268No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC61440.269400426199893No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG30010.13158702458103497No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26610.11667879787075443No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC25690.11264480711385501No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG25650.11246941621138112No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74500.086.210741
CGTAGGG5950.077.4510352
AGGGATG45750.075.809965
TAGGGCA14150.075.392894
CTACGAC11400.073.379745
CGAAGGG21250.072.1401142
ACGGGTA3350.071.5461654
AGGGAAT27900.070.409795
GGATGGC36450.070.3973167
GAATAGG15400.069.968051
GGTAAGG12650.069.928191
TCCGCTA12350.069.7218861
AGAGGGC32150.068.849523
ACGACCA11950.068.822617
TAGAGGG41950.068.602132
AGTAAGG13200.068.440351
GAGGGAT31500.068.181514
AGGGTAC10650.067.956675
TACGACC12350.067.735146
ATAGGGA23550.067.450753
Produced by FastQC (version 0.11.5)