FastQCFastQC Report
Mon 27 Feb 2023
SRR3127316.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127316.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2661149
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316021.1875321524649691No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43550.16365111461252263No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33180.12468298468067741No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC30990.11645345675871588TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG28100.10559348612197213No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG26920.10115931125991065No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT103800.087.119091
TAGGGCA15500.074.903264
CGTAGGG8250.072.9261552
AGGGTAT17500.072.251975
AGGGATG42450.070.755245
AAGGGTA18350.069.417464
TAGGGTA11150.069.1360244
AGGGCAT24700.069.078865
ATAGGGC19750.068.780633
GGTAAGG16150.068.426871
ATAGCGG6000.068.186651
AGTAAGG15700.067.991961
TAGAGGG45450.067.428112
CGAAGGG25950.067.380292
AGTAGGG44300.067.374772
ACTAGGG15550.067.1042252
GAATAGG16700.067.018071
TAGGGTC8100.066.734184
AAGAGGG95800.065.941842
ATGAGGG39600.065.875672
Produced by FastQC (version 0.11.5)