FastQCFastQC Report
Mon 27 Feb 2023
SRR3127315.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127315.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2311876
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250211.0822812296161213No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37260.16116781349864784No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG36930.15974040130179992No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC36500.15788043995439202TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG34690.1500513003292564No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29500.12760199941519354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82000.087.1274641
CGTAGGG6500.079.752352
TAGGGCG3800.076.890434
TAGGGCA13300.073.347094
TAGGGTA8100.072.7259144
AGTAAGG13500.071.610441
GAATAGG12650.070.084761
AGGGATG36400.069.974425
ATAGGGC15850.069.871933
AAGGGTA16100.068.786974
GGTAAGG13450.068.721091
GTAGGGT8800.068.547483
AGGGCAT23900.067.443115
ATAGCGG5050.067.235311
ACGGGTA1900.066.9690864
TAAGGGT9450.066.824723
AGGGTAC9500.066.2861255
AAGAGGG79250.065.8282852
CGAAGGG22750.065.666232
ACTAGGG12050.065.3120962
Produced by FastQC (version 0.11.5)