FastQCFastQC Report
Mon 27 Feb 2023
SRR3127314.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127314.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2355582
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240781.022167770003337No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG39260.16666794023727471No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36820.1563095659586463No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC36360.15435675769300328TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG35310.149899260564905No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29440.12497972900115556No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80950.086.235791
CGTAGGG7300.076.660572
AATGCGG5500.074.459441
ACGGGTA2400.074.410524
AGGGATG37450.073.035375
TAGGGCA14400.072.452364
AGGGTAT14300.070.6585165
TAGACGG3550.070.27661
GAATAGG12050.069.533841
TAGGGTA10000.069.084314
AGGGCAT20450.068.942975
ATAGCGG5100.067.3776551
TAGCGGG12450.067.235492
AGTAAGG13250.067.14431
AGTAGGG36350.066.885822
TAGAGGG38900.066.4907152
CGAAGGG23550.065.698122
ATGAGGG32600.065.058932
GAGGGAT30500.064.870064
GTAGGGA18400.064.6196753
Produced by FastQC (version 0.11.5)