FastQCFastQC Report
Mon 27 Feb 2023
SRR3127312.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127312.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2571439
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295601.1495508934880432No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92900.085.5825651
TAGGGCA12400.076.408184
AGTAAGG16500.072.878541
ATAGGGC16800.072.389563
AGTAGGG42900.071.640042
CGTAGGG6200.071.466362
TATAGGG20150.070.881532
ACGGGTA3300.069.9918754
GAATAGG15900.069.697021
TAGAGGG47800.069.621332
ATAGGGA26150.068.8584443
AGGGTAT15050.068.0703055
AGGGATG39050.067.03045
ATAGAGG18200.066.589411
AGAGGGC39850.066.004173
AGGGCAT20600.065.927755
ATAGCGG4750.065.5231
GTAGGGT8850.065.512983
AGATAGG14300.065.293891
GGTAAGG14750.065.220121
Produced by FastQC (version 0.11.5)