FastQCFastQC Report
Mon 27 Feb 2023
SRR3127311.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127311.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619460
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287831.0988142594275156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95900.086.709491
ATAGGGC15250.074.8852163
CGTAGGG6600.072.6674352
TAGGGCA13100.070.673174
AGTAGGG43700.069.507972
GTAGGGT9850.069.181733
TATAGGG20500.068.8099062
ATAGCGG5300.068.3696751
AGGGATG37900.068.0758065
GAATAGG15400.067.839231
AGATAGG14650.067.1362841
TAGAGGG49600.067.11742
AGAAGGG98600.066.285942
AGTAAGG17350.065.910621
AGGGTAT12700.065.8682255
ACGGGTA3500.065.794214
TAAGGGA20100.065.466883
GGTAAGG15850.065.319381
ATAGGGA23400.064.870363
AAGAGGG93900.064.5462342
Produced by FastQC (version 0.11.5)