FastQCFastQC Report
Mon 27 Feb 2023
SRR3127310.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127310.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2437354
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290261.190881587163785No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC68490.2810014466507532No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC25770.10572940984362551No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98250.084.7614441
TAGGGCG2950.078.0775154
TAGGGTA11600.075.7766654
AGGGATG42800.072.924965
ACGGGTA2000.072.859074
GAGGGAT36100.071.615564
GTAGGGT12300.071.4627153
CTACGAC12500.070.320755
AGTAGGG40750.070.2477952
ATGCGGG12900.069.9607542
ATAGCGG5250.069.863061
TAGGGCA13000.069.424024
TAAGGGA24950.068.953063
TACGACC12550.068.916946
ATAGGGC16100.068.0256963
TACGAGG5150.067.567341
AGGGCAT23600.067.122765
TCCGCTA13400.067.0256351
ACGACCA12800.066.836447
GAATAGG14000.066.5042651
Produced by FastQC (version 0.11.5)