FastQCFastQC Report
Mon 27 Feb 2023
SRR3127308.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127308.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161174
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230061.0645140095151986No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC60390.27943145716170936No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC34220.1583398652769282No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG32390.149872245362937No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG27170.12571870659188017No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79900.085.1821751
TAGGGTA9000.075.7179954
CGTAGGG5300.075.413282
TAGGGCA10750.075.19584
AGGGATG34250.073.549175
AGTAGGG35050.073.1161352
GTAGGGT9450.072.60973
TAGGGCG3000.072.062644
CTACGAC12300.071.833395
TAAGGGA19300.070.130793
ATAGCGG3300.069.8890841
CGCTACG12650.069.84593
TACGACC12600.069.3770756
AGGGCAT20900.069.259295
TCCGCTA13050.068.889031
ACGACCA12400.068.601017
ATAGGGC12900.068.1279753
ATAAGGG30400.067.7493742
TAGGGAC12250.067.139114
AGTAAGG13850.066.948931
Produced by FastQC (version 0.11.5)