FastQCFastQC Report
Mon 27 Feb 2023
SRR3127307.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127307.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2043648
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275331.347247666917199No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC32170.15741458411624704No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG26850.1313827038707253No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG26500.12967008017036202No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT87800.085.2030261
TAGGGTA8850.075.9529344
ATAACGG1900.071.766821
TAGGGCA10550.071.7340244
ATAAGGG26850.071.4278642
AGGGCAT20600.071.1933065
AGTAGGG33650.070.4039842
GTAGGGC10100.069.3452453
TAGAGGG35750.069.292522
TAAGAGG15400.069.002851
AAGGGTA15450.068.7593464
AGAGGGC27100.068.5140463
TAGCGGG10300.067.998732
AATCGGG3400.067.7437062
AGGGTAT15300.067.282865
AGGGAAT23600.067.122745
AGTAAGG13750.067.024361
TAAGGGA18100.067.002793
AGGGATG25150.066.910895
GTAGGGA16450.066.865453
Produced by FastQC (version 0.11.5)