FastQCFastQC Report
Mon 27 Feb 2023
SRR3127306.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127306.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792644
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220331.2290783892395813No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG37570.2095787005116465No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC37030.20656639020352063No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG33570.1872652908218252No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69500.086.1217961
CGTAGGG4700.075.236662
TAGGGCA7650.074.574444
ACGGGTA1650.074.2943044
TAGGGCG3750.074.184
AGTAAGG9650.071.849921
AGGGTAC9150.071.388465
AGGGCAT14900.070.9627765
ATAAGGG22800.070.722462
TAGGGTA5800.070.722454
ATAGAGG11200.070.329041
AGTAGGG30250.069.982112
AGGGATG23850.069.5537645
TAGAGGG27050.069.0230262
AGGGAAT20250.068.9232565
GGGTACC7400.068.584566
AGGGTAT10350.068.560015
GTAGGGT7900.068.036793
ATAGGGC10650.067.7341843
GTAAAGG14450.067.567641
Produced by FastQC (version 0.11.5)