FastQCFastQC Report
Mon 27 Feb 2023
SRR3127304.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127304.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2273435
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278571.2253264333486553No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29420.12940770244145974No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27820.12236989401500373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86300.084.28151
TAGGGTA6950.073.728794
CGTAGGG5900.073.30462
ATAACGG2450.072.9239651
TAGGGCA11750.072.816354
TGTAGGG18050.071.1018452
ACGGGTC2750.070.088474
AGGGATG33400.069.9528355
ATAGCGG5400.069.654471
AGTAGGG36500.069.163482
GGTAAGG14550.068.505531
TAAGGGA19600.068.357193
GTAGGGT9300.068.2411353
TAGAGGG46450.068.213392
ATAGAGG14950.067.301591
ATAGGGA19400.067.123333
ATAGGGC12700.066.9993063
GATAGGG32850.066.544632
AGATAGG13500.066.520021
AATAGGG31400.066.473532
Produced by FastQC (version 0.11.5)