FastQCFastQC Report
Mon 27 Feb 2023
SRR3127303.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127303.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985764
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230931.162927719507454No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG26590.13390312242542418TruSeq Adapter, Index 13 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25940.13062982308068835No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24870.12524146877473857No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC22710.11436404325992414TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG21910.11033536714332619TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68250.085.847821
TAGGGCA10450.074.8531344
TAAGGGA17050.071.303943
TAGGGTA6350.070.496594
AGTAGGG33100.069.8991242
AGGGTAT12600.069.372765
GGTAAGG13600.069.318931
ATAGGGC12500.068.608763
ATACCGG551.6370905E-1068.562721
AGGGATG27050.068.4503255
TAGAGGG37650.068.210262
AATAGGG30000.067.854822
GAATAGG13350.067.792361
AGGGACT28850.067.437445
TAGCGGG9300.067.388672
AGGGCAT13400.067.335335
TATAGGG16800.067.3162842
ATAGAGG13400.067.187631
AGGGAAT17750.066.9836355
ATAGGGA15400.066.398343
Produced by FastQC (version 0.11.5)