FastQCFastQC Report
Mon 27 Feb 2023
SRR3127302.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127302.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2030440
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223601.1012391402848642No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG27370.13479836882646126TruSeq Adapter, Index 13 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26010.12810031323259982No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24390.12012174701050018No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC23180.11416244754831464TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG23020.11337444100786036TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67400.083.883191
TAGGGCA10450.078.2522664
CGTAGGG5650.075.743572
AGTAGGG34750.069.695582
TAGGGTA6350.068.829284
AGGGATG29350.068.69315
TAGAGGG35050.068.294012
AGGGTAA8650.067.913775
GGTAAGG11800.067.8201451
TGTAGGG13950.067.76022
ATAGGGA16650.067.177943
ATAGGGC12350.066.974533
TATAGGG15200.066.828692
AGATAGG10150.066.786431
TAAGGGA17550.066.678563
ACTAGGG9600.066.622442
AGGGAAT21000.066.466245
AATAGGG27250.066.4426652
TAAGCGG4900.066.289511
ATAGAGG13500.065.905231
Produced by FastQC (version 0.11.5)