FastQCFastQC Report
Mon 27 Feb 2023
SRR3127301.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127301.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2531473
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322191.2727372561350643No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99700.084.233441
TAGGGCA13650.075.417544
GAATAGG17300.074.4736561
CGTAGGG6000.073.644032
ATAGGGA22450.071.400123
ATAGGGC15500.071.268423
ACGGGTA3300.071.2224664
AGGGATG37100.070.573595
TAAGGGA21700.070.401933
AGTAAGG17350.070.193761
AGTAGGG42000.069.8387152
TATAGGG23200.069.294552
AGATAGG16700.068.983911
ATGCGGG15550.068.923192
TAGAGGG47050.068.736872
AATGCGG6900.068.147381
AGGGCAT21800.067.707085
GGAGCTA61600.067.5971159
AAGGGAC24800.067.477554
AATAGGG42500.067.358012
Produced by FastQC (version 0.11.5)