FastQCFastQC Report
Mon 27 Feb 2023
SRR3127300.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127300.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2178692
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263711.2104051421678694No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81900.084.704291
GAATAGG14250.076.118391
TAGGGCA12100.075.179364
ATAGGGC13150.071.3273
AGGGCAT18150.070.943555
AGTAAGG13850.070.4850851
ATAGGGA20900.070.361483
TAGAGGG40900.070.1811752
AGGGATG33300.069.432035
AGTAGGG38400.069.2268452
TATAGGG20500.068.05572
AAGGGAC20000.067.400444
CGTAGGG5950.067.33312
AATAGGG35600.066.4630662
ACGGGTA3200.066.281024
ATGAGGG36100.066.064772
AGGGAAT22000.065.7910845
TAGGGTC6100.065.677374
AGAGGGC31950.065.647143
TAAGGGA17700.065.240833
Produced by FastQC (version 0.11.5)