FastQCFastQC Report
Mon 27 Feb 2023
SRR3127299.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127299.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221978
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256651.1550519402082289No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG22310.10040603462320508No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80950.084.722851
TAGGGCA12350.074.588544
ATAGGGC15500.073.074923
CGTAGGG4950.072.209522
AGTAGGG35900.071.136322
GGTAAGG13850.070.692181
GAATAGG15150.070.529541
AGGGATG32900.069.283355
GTACGGG5450.069.036622
ATAGGGA20300.068.9927143
TAGAGGG40200.068.90892
AGGGCAT19000.068.271355
AATAGGG34600.067.828242
GGAGCTA50550.067.592159
ACGGGTA3350.067.340984
TAAGGGA18500.066.559883
ATAAGGG32050.066.327932
GGATAGG15500.066.203761
AGATAGG14550.065.997121
ATGAGGG37500.065.969092
Produced by FastQC (version 0.11.5)