FastQCFastQC Report
Mon 27 Feb 2023
SRR3127297.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127297.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315704
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT527462.277752251583104No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT26580.11478150920843078No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT25700.11098136894870847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT173000.088.449681
ATAGGGC13350.073.475433
AGGGATG33600.071.467825
TAGGGCA11900.070.539634
ATAGGGA19800.070.4995963
AGTAGGG41100.070.450912
ACGGGTA2700.068.117844
TAAGGGA18700.068.0898363
TAGAGGG40500.067.5356452
TAGGGTC7200.066.807894
GAATAGG13200.066.486651
GTAGGGA17450.066.481343
AGAGGGC34800.066.401363
TATAGGG17700.066.341642
CGTAGGG6500.066.021922
TGTAGGG19250.065.899432
ATAAGGG30450.065.820582
AAGAGGG80900.065.8120652
GGTAAGG13500.065.708191
AAGGGGC73450.065.553224
Produced by FastQC (version 0.11.5)